Significance of Enzymatic assay
Enzymatic assays are laboratory tests measuring enzyme activity or using enzymes to quantify substances. They assess mushroom extract potential, periodontal disease insights, and digestive enzyme levels in fish. These assays measure enzyme activity in cellulose degradation, parasite infections in animals, glucokinase activity, and lipid metabolism. They determine cholesterol levels, evaluate plant extract effects on alpha-amylase, and assess liver function. These procedures detect and quantify specific chemical substances and proteins in biological samples.
Synonyms: Enzyme activity assay, Enzymatic analysis, Biochemical assay, Enzyme measurement, Enzyme assay, Enzyme activity measurement
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Enzymatic assay in scientific sources
Enzymatic assay is a lab procedure using enzymes to detect/quantify substances in a sample. It measures enzyme activity to understand kinetics, function, and diagnose health.
From: International Journal of Pharmacology
(1) Human COX-1 and COX-2 activities were determined as described by Wakitani et al. (1998) using this.[1] (2) The supernatant was frozen and stored for use in subsequent enzymatic assays.[2] (3) CAT activity was detected at 240 nm by monitoring the decomposition of H2O2 and Glutathione peroxidase activity was determined spectrophotometrically.[3] (4) These types of assays have been performed by researchers on sheep and goats infected with parasites.[4] (5) An enzymatic assay was developed to measure glucokinase activity, contributing to the understanding of its kinetics and function.[5]
From: Asian Journal of Pharmaceutics
(1) The assay for PARP 1 activity was performed according to the original method based on estimation of residual NAD + concentration.[6] (2) These are used to analyze all biochemical parameters, following principled methods and operated by an automatic analyzer.[7] (3) A method based on chemical determination of NAD+ used to assess PARP 1 activity in nuclei, adapted to quantify NAD+ consumed by nuclei in 10 minutes.[8] (4) A procedure to measure the activity of enzymes, such as PARP 1, in a sample.[9] (5) A procedure performed with colloidal chitin as the substrate to measure chitinase activity.[10]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) in vitro tests conducted to evaluate the activity of anabaenopeptins against serine proteases and carboxypeptidase A, revealing their inhibitory effects and pharmaceutical potential.[11] (2) Enzymatic assay was used to analyze Urine 8-OHdG, with a LOD of 0.32 ng/mL, to assess oxidative stress.[12] (3) Enzymatic assays were conducted to determine laccase activities at room temperature, with the oxidation of syringaldazine being measured to quantify enzyme activity.[13] (4) Enzymatic assay refers to a procedure for measuring the activity of an enzyme, and the lipase activity was determined using cell-free supernatant in the enzymatic assay.[14] (5) Enzymatic assays were performed to measure protease, total ATPase, AAT, ALAT, and cytochrome-c-oxidase activities in the control and experimental B. mori larval muscle and silkgland.[15]