Significance of Disk diffusion method
The disk diffusion method is a widely used laboratory technique for assessing the susceptibility of bacteria and fungi to various antimicrobial agents. By placing antibiotic-impregnated disks on agar plates inoculated with microorganisms, researchers can measure the zones of inhibition, which indicate the effectiveness of the antibiotics or other substances being tested. This method helps in evaluating the antibacterial activity of different compounds and is essential for determining antibiotic resistance and effectiveness in clinical settings.
Synonyms: Agar diffusion method, Kirby-bauer test, Antibiotic sensitivity test, Antibiotic susceptibility test, Antimicrobial sensitivity test
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The concept of Disk diffusion method in scientific sources
The Disk diffusion method is a standard laboratory technique for assessing the susceptibility of microorganisms, including E. alsinoides extracts and silver nanoparticles, by measuring the zone of inhibition on agar plates.
From: The Malaysian Journal of Medical Sciences
(1) A laboratory technique used to test the susceptibility of bacteria to antibiotics by measuring the inhibition zones around antibiotic-impregnated disks.[1] (2) A laboratory technique to test the susceptibility of bacteria to antibiotics by observing the effectiveness of antibiotic-impregnated disks on a bacterial lawn.[2]