Significance of Cytotoxicity assay
Cytotoxicity assays are laboratory tests used to assess a substance's harmful effects on cells. These assays determine the degree to which a substance can damage or kill cells, often employing methods like the MTT assay to measure cell viability. They are used to evaluate the safety of substances like drugs, plant extracts, and other compounds, especially in cancer research and drug development, ensuring they do not harm healthy cells while targeting the harmful ones.
Synonyms: Cytotoxic assay, Cell viability assay, Toxicity testing, Cytotoxicity testing, Cell death assay
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'Cytotoxicity assay'
In Hinduism, a cytotoxicity assay, a short-term test, could assess a polyherbal formulation's biological activities. This might align with Ayurvedic principles, evaluating herbal remedies' impact on cellular health and potential therapeutic effects, reflecting the holistic approach to well-being.
From: Journal of Ayurvedic and Herbal Medicine
(1) This is a short-term assay used to evaluate the biological activities of the polyherbal formulation, as mentioned in the provided text material.[1]
The concept of Cytotoxicity assay in scientific sources
Cytotoxicity assay is a laboratory procedure used to assess the toxicity of a substance on cells. It evaluates a substance's ability to damage or kill cells, often measuring cell viability or the effects on cell survival. These assays are crucial for evaluating potential drugs, extracts, and other compounds.
From: International Journal of Pharmacology
(1) This is a method that determines the cytotoxicity of compounds on BAE-1 cells using trypan blue dye exclusion.[2] (2) Serial dilutions were made to obtain different concentrations of fractions and concentrations for in vitro this.[3] (3) A test performed after cells grow in media to 60-80% confluence, using the MTT method.[4] (4) This was employed to evaluate cytotoxicity of the hydrazide derivative against HepG2 and Bel-7402 cell lines.[5] (5) This assay and valuation of inflammatory markers were done on a microplate reader.[6]
From: Asian Journal of Pharmaceutics
(1) In this assay, the rate of HSC growth inhibition increases and viability of AFB-treated cells decreased whenever the aflatoxin concentration was high.[7] (2) This is a procedure used to determine the cytotoxic effect of a compound, by measuring the viability of cells after treatment with different concentrations of the compound.[8] (3) The results of this are presented in Table 1 and Figure 1.[9] (4) These are used to determine EGC -3-gallate PEGylated- PLGA NPs.[10] (5) A rapid colorimetric assay is used for cellular growth and survival, which can be applied to proliferation and this.[11]
From: The Malaysian Journal of Medical Sciences
(1) This is a method used to examine the potential harmful effects of substances on cells, relying on the conversion of a tetrazolium salt to a colored product.[12] (2) This is a colorimetric microtiter assay, specifically the MTT assay, used to determine the oncolytic effects of NDV strains by measuring their ability to kill tumor cells.[13] (3) A method to determine the toxic effects of the extracts on human cell lines, ensuring that while acting against malaria, the extracts remain non-toxic to human cells.[14] (4) An experimental procedure to determine the toxicity of a substance by assessing its effects on living cells.[15] (5) Tests conducted to evaluate the toxic effects of substances on living cells, particularly their effects on cell viability.[16]
From: Journal of Medicinal Plants for Economic Development
(1) The cytotoxicity assay of the oils was also assessed by using human dermal fibroplast (MRHF) cell lines.[17] (2) It is a method used to determine the number of dead and surviving cells.[18] (3) This is a test used to assess the toxic effects of substances on cells, which is important for evaluating the safety of medicinal plant extracts and compounds.[19]
From: Onderstepoort Journal of Veterinary Research
(1) Cytotoxicity assays are used to determine the effect of geigerin on C2C12 cell cultures, using methods like MTT and LDH assays and flow cytometry.[20]
From: International Journal of Pharmacology
(1) A cytotoxicity assay is a method used to measure the toxic effect of substances on cells, often by assessing cell viability or number after exposure.[21] (2) These are tests used to measure the toxicity of substances towards cells, often employing rapid colorimetric methods.[22] (3) Tests, such as the MTT assay, used to assess the toxicity of compounds towards cells.[23] (4) A method used to measure the toxic effects of drugs on cells, such as the MTT assay, employed here to determine the IC50 values for verapamil in different cell lines.[24] (5) These are laboratory tests used to measure the degree to which a substance can kill cells.[25]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) Cytotoxicity assays using human epithelial A 549 lung model cell cultures did not exhibit any correlation of 48 h cytotoxic responses with either PAH concentration or specific isomer content for the soot PM species listed.[26] (2) Cytotoxicity assay is a method used to examine the harmful effects of ultraviolet A light and sex hormones on Jurkat T cells, measuring cell death and viability.[27] (3) This is a method used to assess the toxic effects of substances on cells, typically by measuring cell viability and death.[28] (4) Cytotoxicity assays, along with proliferation assays, are applications of a rapid colorimetric assay used for assessing cellular growth and survival.[29]
From: Sustainability Journal (MDPI)
(1) Tests affected by nanofibrous materials, indicating a need for careful consideration when using these materials in applications involving cell viability assessment.[30]