Significance of Column chromatography
Column chromatography is a laboratory technique used extensively in various fields to separate and purify compounds from mixtures. It leverages the differing affinities of compounds for a stationary phase and a mobile phase, allowing for their separation based on properties like polarity. This technique is employed in Ayurveda, science, and health sciences to isolate, purify, and analyze specific components, such as flavonoids, steroids, and phytoconstituents, from extracts of various materials. It is a versatile method utilized in the extraction and synthesis processes for a wide range of applications.
Synonyms: Column chromatography technique, Chromatographic column, Liquid chromatography, Separation technique, Chromatographic method, Analytical technique, Purification process
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'Column chromatography'
In Hinduism, column chromatography symbolizes the purification process. Like separating mixture components, it extracts and refines, potentially representing the journey to separate the pure self (Atman) from the impurities of worldly existence.
From: Journal of Ayurvedic and Herbal Medicine
(1) This technique is used to separate and purify the different components of a mixture, and is used in the extraction process.[1]
The concept of Column chromatography in scientific sources
Column chromatography is a lab technique used for separating and purifying compounds from mixtures. It separates compounds based on their interactions with a stationary phase, allowing for the isolation of specific components, such as flavonoids, pigments, or active compounds, from plant extracts and synthesized products.
From: International Journal of Pharmacology
(1) A purification technique performed on an alumina with gradient hexane with increasing polarity with ethyl acetate.[2] (2) (CC) was carried out over silica gel 60 (70-230 and 230-400 mesh, Merck) according to the text.[3] (3) A separation technique was used to isolate Zerumbone, with a flow rate of approximately 50 mL/hr.[4] (4) This technique was carried out using silica gel 60 (0.063-0.200 mm) and silica gel 60 (particle size less than 0.063 mm) for separation purposes.[5] (5) After extraction and evaporation, 430 g of the dichloromethane extract were subjected to this process, then eluted with a mixture.[6]
From: Asian Journal of Pharmaceutics
(1) This method was employed to isolate heptamethoxy flavones from S. amaranthoides for further study.[7] (2) It is a method for separation and purification of solids and liquids, and it was used with silica gel.[8] (3) Thus, a column chromatography was chalked out to separate and isolates the compounds. Using n-Hexane:ethyl acetate (90:10) as eluent, three fractionates were eluted and with n-Hexane:DCM (90:10), two fractionates were eluted.[9] (4) The condensed ethanol extract of whole plant was subjected to this process.[10] (5) A purification technique used to isolate the product using ethyl acetate and hexane as the mobile phase.[11]
From: The Malaysian Journal of Medical Sciences
(1) Column chromatography is a purification technique used to separate the dichloromethane extract from bamboo shoots, employing silica gel and gradient elution with chloroform, ethyl acetate, and methanol.[12] (2) A laboratory technique utilized for separating and isolating compounds from extracts based on their chemical properties.[13] (3) A laboratory technique used to separate and purify the compounds from plant extracts, including those from bamboo shoots.[14]
From: Journal of Medicinal Plants for Economic Development
(1) This method was used to isolate the antifungal compounds from the acetone extract of Lonchocarpus capassa, using silica gel as a stationary phase and various eluent solvent systems.[15] (2) This is a separation technique used to isolate compounds from the ethyl acetate fraction, based on their different affinities for the stationary phase in a column.[16] (3) A separation technique used to isolate and purify compounds from a mixture, based on their different physical and chemical properties.[17]
From: Onderstepoort Journal of Veterinary Research
(1) A method used to isolate toxic fractions from an extract prepared from sorghum beer residue.[18] (2) This is a method used to isolate mesembrine from a methanol extract, which is a technique to separate and purify compounds from the Sceletium tortuosum plant.[19]
From: Sustainability Journal (MDPI)
(1) Separation was attempted by implementing column chromatography with a silica gel stationary phase and ethanol mobile phase followed by water due to the affinity of levulinic acid toward ethanol.[20] (2) Various fractions were separated by column chromatography on silica gel using different eluent systems to obtain combined fractions and subfractions, which were further purified.[21] (3) Column chromatography on silica gel was used to further separate the crude extract into four fractions by column chromatography on silica gel using as eluent a mixture of hexane:diethyl ether 7:3.[22] (4) Column chromatography of leaf pigments is revisited as a simple and ecofriendly separation of plant carotenoids, chlorophylls, and flavonoids from green and red leaves.[23]
From: International Journal of Pharmacology
(1) A laboratory technique used to separate mixtures of compounds, employed in the purification of parameritannins.[24] (2) This separation technique was utilized to purify the ethyl acetate extract using silica gel and a gradient elution system.[25] (3) A laboratory technique used to separate compounds within a mixture based on their differing affinities for a stationary phase and a mobile phase.[26] (4) A technique used to separate compounds from extracts based on their properties, applied to the dichloromethane leave extract.[27]