Significance of Chromatography
Chromatography is a versatile laboratory technique for separating mixtures. It separates components based on their behavior in a stationary phase, facilitated by a mobile phase. Chromatography is used in various fields including Ayurveda, science, and health sciences. It is employed in drug standardization, quality assessment, and analysis of compounds. The technique is used to separate, analyze, and quantify components, including those in herbal drugs, biological samples, and synthesized compounds. Different methods like HPLC, TLC, and column chromatography are used, often combined with other analytical techniques.
Synonyms: Separation, Purification, Analysis, Fractionation, Partitioning, Detection, Identification
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
Hindu concept of 'Chromatography'
In Hinduism, chromatography standardizes herbal drugs, crucial for assessing quality. It's used in studying Dhutturadi Kera Taila and Dhutturadi Taila, using High-Performance Thin-Layer Chromatography (HPTLC) for analysis and quality evaluation of traditional herbal medicines.
From: Journal of Ayurveda and Integrated Medical Sciences
(1) This is an important tool for standardizing herbal drugs, utilized in the study for the quality assessment of Dhutturadi Kera Taila and Dhutturadi Taila, involving the use of HPTLC.[1]
The concept of Chromatography in scientific sources
Chromatography is a versatile laboratory technique for separating and analyzing mixtures. It uses stationary and mobile phases to separate components based on their physical/chemical properties. Applications include analyzing herbal medicines, drug interactions, and purifying compounds, with methods like TLC, HPLC, and column chromatography being commonly employed.
From: Asian Journal of Pharmaceutics
(1) An analytical method used for the estimation of luliconazole and gallic acid.[2] (2) It is the process that was not affected by variations in wavelength, variations in mobile phase flow rate, and variations in column oven temperature.[3] (3) The process by which the HPLC method for simultaneous estimation of metformin and fenofibrate was developed with better reproducibility.[4] (4) A separation technique used to isolate components of a mixture.[5] (5) It is a method used for quantitative determination of mianserin, including high-performance liquid chromatography.[6]
From: International Journal of Pharmacology
(1) This method was used to give 6 fractions which overlapping degrees were evaluated to be less than 10%.[7] (2) It is one of the more widely used analytical procedures for the determination of Kanamycin levels.[8] (3) This is a laboratory technique for the separation of mixtures, commonly used in analytical chemistry to identify and quantify components.[9] (4) Chromatography on a silica gel column was employed to further separate and obtain active compounds from fraction F4.[10] (5) The chromatographic system utilized included a Waters 600 pump, a Waters 486 UV detector, and a reversed-phase C18 column.[11]
From: The Malaysian Journal of Medical Sciences
(1) It is a method of purification, specifically immunoaffinity chromatography, that is rapid, simple, specific, and can be easily scaled up.[12]
From: International Journal of Pharmacology
(1) This separation technique, including methods like HPLC, was initially used for paclitaxel extraction but faced challenges with purity and yield without pre-purification.[13]
From: International Journal of Environmental Research and Public Health (MDPI)
(1) It is a technique used to analyze samples, separating and identifying the different components, including pharmaceuticals, present in sewage sludge.[14]