Significance of CDNA
cDNA, or complementary DNA, is synthesized from RNA templates. This process, often involving reverse transcriptase, creates a DNA molecule used in various molecular biology techniques. cDNA serves as a template for PCR amplification and is crucial in gene expression analysis, including RT-PCR and qRT-PCR. These techniques utilize cDNA to study and quantify gene expression levels.
Synonyms: Complementary dna
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The concept of CDNA in scientific sources
From: International Journal of Pharmacology
(1) Complementary DNA, synthesized from RNA and used as a template in Real-time PCR.[1] (2) cDNA is synthesized from RNA and serves as the template for DNA amplification in techniques like qRT-PCR.[2] (3) cDNA, or complementary DNA, is synthesized from RNA templates and is used in techniques like RT-qPCR to analyze gene expression.[3] (4) Complementary DNA synthesized from RNA using a transcription process, often involving Oligo (dT) and SuperScrip First-Strand Synthesis System.[4] (5) RNA was converted into cDNA using the molony murine leukaemia virus reverse transcriptase for PCR analysis.[5]
From: The Malaysian Journal of Medical Sciences
(1) This was obtained through reverse transcription of the total RNA extracted from the blood plasma and was used to measure the expression of miR-141.[6] (2) cDNA is the complementary DNA that is reverse transcribed from RNA and used in several diagnostic techniques, including microarray and biosensor-based methods.[7] (3) Complementary DNA synthesized from an mRNA template, used in cloning and expression of genes.[8]
From: Asian Journal of Pharmaceutics
(1) It is synthesized from RNA and used for evaluating gene expression.[9]
From: International Journal of Pharmacology
(1) cDNA is synthesized from RNA using reverse transcription kits for use in PCR amplification.[10] (2) One microgram of RNA was converted to cDNA by AMV reversed transcriptase.[11] (3) cDNA, or complementary DNA, is synthesized from RNA and used as a template for PCR amplification.[12] (4) Complementary DNA synthesized from total RNA, used as a template for real-time RT-PCR analysis.[13] (5) Complementary DNA, synthesized from RNA using a kit, which is then used for PCR analysis.[14]