Significance of Affinity Chromatography
Affinity chromatography is a purification technique that leverages specific interactions between proteins and ligands to isolate desired proteins from complex mixtures. This method is particularly effective for purifying proteins like lectins from various sources, utilizing reversible interactions for separation. It can be applied in the analysis of pharmaceutical and biological substances and is instrumental in extracting proteins through their unique binding properties. Overall, affinity chromatography plays a crucial role in laboratory techniques aimed at isolating biological molecules.
Synonyms: Protein purification
The below excerpts are indicatory and do represent direct quotations or translations. It is your responsibility to fact check each reference.
The concept of Affinity Chromatography in scientific sources
Affinity Chromatography is a purification technique that utilizes the specific binding of ligands, like lectins to carbohydrates, to isolate proteins effectively. It enhances extraction processes, exemplified by its application with guar-gum for selective protein separation.
From: The Malaysian Journal of Medical Sciences
(1) This is a purification method used to purify the rLipL32 protein using a HisTrap FF crude column and an AKTA Pure Purification System.[1] (2) A laboratory technique used for the separation of biological molecules based on their specific interactions.[2]